Molecular Biology

Sequencer

Schedule an Instrument

CORE Director: Rob Miller, PhD
Manager: Melissa Sanchez, PhD
Bioinformatics Specialist: Marijan Posavi, PhD

Phone: (505) 277-4278

The Molecular Biology Core located within the Department of Biology at the University of New Mexico, provides genetic sequencing services to University investigators and maintains a repository of equipment for use by faculty, staff and students to carry out molecular biology experiments.  A Bioinformatics Specialist is also on staff to assist with data analysis.

Sanger Sequencing and DNA Fragment Analysis

Bioinformatics

Services Offered by CETI’s Bioinformatics Support Group

  • RNASeq: Differential gene expression analysis, de novo transcriptome assembly and annotation.
  • Genome/DNA Sequencing: De novo genome assembly, annotation, genome variation analysis.
  • High-Throughput Data Analysis: Improved efficiency using CETI high-performance computer servers (24 cores/48 threads CPUs + 128 GB RAM; 20 cores/40 threads CPUs + 1 TB RAM), and parallel computing on computer clusters (120 nodes/960 cores with 32 GB RAM/core) at the UNM Center for Advanced Research Computing (CARC).
  • Maintain the local Bioinformatics cloud based on the Galaxy platform (developed by Galaxy team of the Penn State University) - a web server containing over 700 different Bioinformatics tools. As a collaboration project with UNM CARC, the server named Pluto (http://pluto.alliance.unm.edu) is open to UNM researchers and collaborators for free. We offer hands on workshops at CARC regularly for new users as well as online workshop materials.
  • Data processing, statistics and visualization using Unix/Linux shell, R, Perl, HTML and Python.

In addition, we are happy to discuss with potential users any special bioinformatics needs they may have with respect to next-gen sequencing, metagenomics analyses, environmental DNA surveys or molecular phylogenetic reconstruction. 

UNM Cloud

Fees

  • Bioanalyzer-High-Sensitivity-Analysis

$95.00

  • Bioanalyzer-Nano-RNA-Analysis

$65.00

  • Bioinformatic Support Per Hour Fee

$50.00

  • Covaris_Tube

$10.00

  • Digital Print from Gel Doc

$0.25

  • Kapa_DNA-library

$82.00

  • Kapa-RNA-Seq-Library

$75.00

  • Qubit Sample

$1.50

  • Pippin_castette

$60.00

  • qPCR-Analysis

$117.00

  • Sequence or Microsat Sample 3130 Run

$1.00

General Use Equipment

ABI 3130-XL Sanger Sequencer (2)

Bio-Rad CFX96 RT-PCR (2)
MJ Research Tetrad Thermocycler

BioRad ChemiDoc Touch
BioRad GelDoc XR+    
Kodak Image Station 440

Beckman J2-HS Preparative Centrifuge
Beckman L-70 Ultra-Centrifuge
Eppendorf 5810 Refrigerated Tabletop Centrifuge (2)
Jouan CR412 Swinging Bucket Centrifuge
Labconco Centrivap Concentrator (2)

Agilent 2100 Bioanalyzer
Qubit 3.0 Fluorometer
Nanodrop 1000 Spectrophotometer
Nanodrop 2000c Spectrophotometer (2)
Beckman 640B Spectrophotometer

Qiagen TissueLyser II
Covaris M220 Focused-ultrasonicator
Blue Pippin Fragment Collector
Digilab DNA Shearer

Airclean PCR Workstations (2)
Milli-Q Plus Water System
Casy DT Cell Counter
Robbins Scientific Hydra96 Multichannel Dispenser
Orbital shakers and Incubators
Dell workstations for DNA analysis

How to cite core support in your publications

 "Research reported in this publication was supported in part by the National Institute of General Medical Sciences of the National Institutes of Health under award number P30 GM110907.  The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health."